Journal of Pathology and Translational Medicine

Original Articles

Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea: Yosep Chong, Soon Auck Hong, Hoon Kyu Oh, Soo Jin Jung, Bo-Sung Kim, Ji Yun Jeong, Ho-Chang Lee, Gyungyub Gong; J Pathol Transl Med. 2023;57(5):251-264. Published online August 24, 2023; DOI: https://doi.org/10.4132/jptm.2023.07.17

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Abstract PDF Supplementary Material: Background
The Korean Society for Cytopathology introduced a digital proficiency test (PT) in 2021. However, many doubtful opinions remain on whether digitally scanned images can satisfactorily present subtle differences in the nuclear features and chromatin patterns of cytological samples.
Methods
We prepared 30 whole-slide images (WSIs) from the conventional PT archive by a selection process for digital PT. Digital and conventional PT were performed in parallel for volunteer institutes, and the results were compared using feedback. To assess the quality of cytological assessment WSIs, 12 slides were collected and scanned using five different scanners, with four cytopathologists evaluating image quality through a questionnaire.
Results
Among the 215 institutes, 108 and 107 participated in glass and digital PT, respectively. No significant difference was noted in category C (major discordance), although the number of discordant cases was slightly higher in the digital PT group. Leica, 3DHistech Pannoramic 250 Flash, and Hamamatsu NanoZoomer 360 systems showed comparable results in terms of image quality, feature presentation, and error rates for most cytological samples. Overall satisfaction was observed with the general convenience and image quality of digital PT.
Conclusions
As three-dimensional clusters are common and nuclear/chromatin features are critical for cytological interpretation, careful selection of scanners and optimal conditions are mandatory for the successful establishment of digital quality assurance programs in cytology.

Current status of cytopathology practice in Korea: impact of the coronavirus pandemic on cytopathology practice: Soon Auck Hong, Haeyoen Jung, Sung Sun Kim, Min-Sun Jin, Jung-Soo Pyo, Ji Yun Jeong, Younghee Choi, Gyungyub Gong, Yosep Chong; J Pathol Transl Med. 2022;56(6):361-369. Published online October 27, 2022; DOI: https://doi.org/10.4132/jptm.2022.09.21

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Background
The Continuous Quality Improvement program for cytopathology in 2020 was completed during the coronavirus pandemic. In this study, we report the result of the quality improvement program.
Methods
Data related to cytopathology practice from each institute were collected and processed at the web-based portal. The proficiency test was conducted using glass slides and whole-slide images (WSIs). Evaluation of the adequacy of gynecology (GYN) slides from each institution and submission of case glass slides and WSIs for the next quality improvement program were performed.
Results
A total of 214 institutions participated in the annual cytopathology survey in 2020. The number of entire cytopathology specimens was 8,220,650, a reduction of 19.0% from the 10,111,755 specimens evaluated in 2019. Notably, the number of respiratory cytopathology specimens, including sputum and bronchial washing/ brushing significantly decreased by 86.9% from 2019, which could be attributed to the global pandemic of coronavirus disease. The ratio of cases with atypical squamous cells to squamous intraepithelial lesions was 4.10. All participating institutions passed the proficiency test and the evaluation of adequacy of GYN slides.
Conclusions
Through the Continuous Quality Improvement program, the effect of coronavirus disease 2019 pandemic, manifesting with a reduction in the number of cytologic examinations, especially in respiratory-related specimen has been identified. The Continuous Quality Improvement Program of the Korean Society for Cytopathology can serve as the gold standard to evaluate the current status of cytopathology practice in Korea.

Citations

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A stepwise approach to fine needle aspiration cytology of lymph nodes
Yosep Chong, Gyeongsin Park, Hee Jeong Cha, Hyun-Jung Kim, Chang Suk Kang, Jamshid Abdul-Ghafar, Seung-Sook Lee
Journal of Pathology and Translational Medicine.2023; 57(4): 196. CrossRef
Diagnostic proficiency test using digital cytopathology and comparative assessment of whole slide images of cytologic samples for quality assurance program in Korea
Yosep Chong, Soon Auck Hong, Hoon Kyu Oh, Soo Jin Jung, Bo-Sung Kim, Ji Yun Jeong, Ho-Chang Lee, Gyungyub Gong
Journal of Pathology and Translational Medicine.2023; 57(5): 251. CrossRef

Prognostic Significance of Aquaporin 5 Expression in Non-small Cell Lung Cancer: Young Min Jo, Tae In Park, Hwa Young Lee, Ji Yun Jeong, Won Kee Lee; J Pathol Transl Med. 2016;50(2):122-128. Published online February 8, 2016; DOI: https://doi.org/10.4132/jptm.2015.10.31

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Abstract PDF

Background
Aquaporins are water channel proteins that play a major role in the movement of water in various human tissues. Recently, it has been found that aquaporins have influence in the carcinogenesis of human malignancies. We analyzed the prognostic impact of aquaporin 5 (AQP5) in non-small lung cancer (NSCLC). Methods: Seventy-six cases of NSCLC were studied, including 44 cases of adenocarcinoma (ADC) and 32 cases of squamous cell carcinoma (SQCC). Tissue microarray was constructed and immunohistochemical staining for AQP5 was performed. Results: AQP5 was positive in 59.2% of the total enrolled NSCLCs (63.7% in ADC and 53.1% in SQCC). The difference in expression of AQP5 according to the histologic grade of the tumor was significant (p<.047), but not in a serial order. When ADC and SQCC were separately evaluated, no significant difference was observed according to the histologic grade of the tumor (p=.076 in ADC and p=.631 in SQCC). No difference was observed between AQP5 expression and other demographic data and tumor characteristics. Disease-free survival (DFS) was higher in AQP5 negative cases than positive cases in ADC (p=.047), but no significance was found in SQCC (p=.068). We were unable to find a significance between AQP5 overexpression and overall survival in either ADC (p=.210) or SQCC (p=.533). Conclusions: AQP5 expression is associated with DFS in ADC of the lung and tumor grade of NSCLC. The present study suggests that AQP5 can be a prognostic factor of NSCLC.

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Aquaporin-mediated dysregulation of cell migration in disease states
Ian M. Smith, Shohini Banerjee, Allison K. Moses, Kimberly M. Stroka
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The Role of Aquaporin 5 (AQP5) in Lung Adenocarcinoma: A Review Article
Lukasz Jaskiewicz, Anna Romaszko-Wojtowicz, Anna Doboszynska, Agnieszka Skowronska
Cells.2023; 12(3): 468. CrossRef
Aquaporins 1, 3 and 5 in Different Tumors, their Expression, Prognosis Value and Role as New Therapeutic Targets
Mahdieh-Sadat Moosavi, Yalda Elham
Pathology & Oncology Research.2020; 26(2): 615. CrossRef
Aquaporins in lung health and disease: Emerging roles, regulation, and clinical implications
Ekta Yadav, Niket Yadav, Ariel Hus, Jagjit S. Yadav
Respiratory Medicine.2020; 174: 106193. CrossRef
Combined Systematic Review and Transcriptomic Analyses of Mammalian Aquaporin Classes 1 to 10 as Biomarkers and Prognostic Indicators in Diverse Cancers
Pak Hin Chow, Joanne Bowen, Andrea J Yool
Cancers.2020; 12(7): 1911. CrossRef
Prognostic Role of S100A8 and S100A9 Protein Expressions in Non-small Cell Carcinoma of the Lung
Hyun Min Koh, Hyo Jung An, Gyung Hyuck Ko, Jeong Hee Lee, Jong Sil Lee, Dong Chul Kim, Jung Wook Yang, Min Hye Kim, Sung Hwan Kim, Kyung Nyeo Jeon, Gyeong-Won Lee, Se Min Jang, Dae Hyun Song
Journal of Pathology and Translational Medicine.2019; 53(1): 13. CrossRef
Effect of FGF/FGFR pathway blocking on lung adenocarcinoma and its cancer‐associated fibroblasts
Ahmed E Hegab, Mari Ozaki, Naofumi Kameyama, Jingtao Gao, Shizuko Kagawa, Hiroyuki Yasuda, Kenzo Soejima, Yongjun Yin, Robert D Guzy, Yoshikazu Nakamura, David M Ornitz, Tomoko Betsuyaku
The Journal of Pathology.2019; 249(2): 193. CrossRef
Anti-cancer effect of Aquaporin 5 silencing in colorectal cancer cells in association with inhibition of Wnt/β-catenin pathway
Wei Wang, Qing Li, Tao Yang, Dongsheng Li, Feng Ding, Hongzhi Sun, Guang Bai
Cytotechnology.2018; 70(2): 615. CrossRef
Knockdown of aquaporin-5 sensitizes colorectal cancer cells to 5-fluorouracil via inhibition of the Wnt–β-catenin signaling pathway
Qing Li, Tao Yang, Dongsheng Li, Feng Ding, Guang Bai, Wei Wang, Hongzhi Sun
Biochemistry and Cell Biology.2018; 96(5): 572. CrossRef
Implications of KRAS mutations in acquired resistance to treatment in NSCLC
Marzia Del Re, Eleonora Rofi, Giuliana Restante, Stefania Crucitta, Elena Arrigoni, Stefano Fogli, Massimo Di Maio, Iacopo Petrini, Romano Danesi
Oncotarget.2018; 9(5): 6630. CrossRef

Brief Case Report

Signet-Ring Cell Carcinoma of the Distal Common Bile Duct: Report of a Case: Hyung Jun Kwon, Ghil-Suk Yoon, Yong Chul Kwon, Sang Geol Kim, Ji Yun Jeong; Korean J Pathol. 2014;48(4):315-318. Published online August 26, 2014; DOI: https://doi.org/10.4132/KoreanJPathol.2014.48.4.315

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Citations

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Rare histotypes of epithelial biliary tract tumors: A literature review
Elena Sapuppo, Oronzo Brunetti, Dalila Tessitore, Giovanni Brandi, Nicola Di Giovanni, Guido Fadda, Claudio Luchini, Maurizio Martini, Davide Quaresmini, Antonio Russo, Mariacarmela Santarpia, Aldo Scarpa, Mario Scartozzi, Giovanni Tuccari, Tindara Franch
Critical Reviews in Oncology/Hematology.2023; 181: 103892. CrossRef
Primary signet-ring cell carcinoma of the extrahepatic bile duct: A case report
Chao-Bang Xie, Yang Wu, Feng Li, Kai-Fei Zhao, Rong-Shu Shi, Qiong Huang, Jin Ao, Di Ke
World Journal of Gastrointestinal Oncology.2022; 14(7): 1356. CrossRef
Duodenal Stenosis Due to Carcinoma of the Lower Bile Duct: A Case Report
Takumi Maki, Atsushi Irisawa, Kenji Notohara, Goro Shibukawa, Ai Sato, Akane Yamabe, Yoshitsugu Yoshida, Shogo Yamamoto, Nobutoshi Soeta, Takuro Saito
Clinical Medicine Insights: Case Reports.2020; 13: 117954762091945. CrossRef
Rapidly Progressing Primary Extrahepatic Bile Duct Signet-Ring Cell Carcinoma in a Caucasian Woman
Jessemae L. Welsh, Omar Jaber, Marina Ivanovic, Frederick C. Johlin, Rami G. El Abiad, Gerald H. Clamon, Mark C. Smith, Carlos H. F. Chan
Journal of Gastrointestinal Cancer.2018; 49(1): 63. CrossRef

Case Study

Fine-Needle Aspiration Cytology of Low-Grade Cribriform Cystadenocarcinoma with Many Psammoma Bodies of the Salivary Gland: Ji Yun Jeong, Dongbin Ahn, Ji Young Park; Korean J Pathol. 2013;47(5):481-485. Published online October 25, 2013; DOI: https://doi.org/10.4132/KoreanJPathol.2013.47.5.481

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Abstract PDF

Low-grade cribriform cystadenocarcinoma (LGCCC) is a rare salivary gland tumor that was recently defined as a variant of cystadenocarcinoma by the 2005 World Health Orgazniation (WHO) classification system. We report cytologic findings of an unusual case of LGCCC with many psammoma bodies. A 90-year-old man presented a palpable mass on his left parotid gland. Fine-needle aspiration (FNA) cytology showed tumor cells that were arranged in clusters and dispersed individually. The tumor cells showed mild atypia and had clear or dense cytoplasm with some vacuoles. Numerous psammoma bodies were noted. After surgical resection, the histologic examination revealed a mixed solid and cystic mass showing intraductal growth with focal stromal invasion. The S-100 protein expressed in the tumor cells, but smooth muscle actin and p63 were positive only in myoepithelial cells. Although LGCCCs resemble other salivary gland tumors, differentiating LGCCC during preoperative FNA is important to avoid unnecessary overtreatment.

Citations

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Salivary Gland Intraductal Carcinoma: How Do 183 Reported Cases Fit Into a Developing Classification
Lester D.R. Thompson, Justin A. Bishop
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Duct tales of a parotid gland swelling
Swati Raj, Monika Singh, Mamta Gupta, Naveen Thapliyal
Cytojournal.2023; 20: 22. CrossRef
Intraductal carcinoma of the parotid gland
Yukiya HIRATA, Kayoko HIGUCHI, Toshitaka NAGAO, Yoko ZUKERAN, Takao KINJO, Naoki WADA
The Journal of the Japanese Society of Clinical Cytology.2022; 61(6): 431. CrossRef
Intraductal carcinoma of the retromolar trigone found with elevated serum CEA and CA19-9 levels: a case report
Mao KAWAKAMI, Nobuhiro UEDA, Yuka TAKAHASHI, Sho ARIKAWA, Nobuhiro YAMAKAWA, Tadaaki KIRITA
Japanese Journal of Oral and Maxillofacial Surgery.2021; 67(5): 292. CrossRef
Endoscopic trans‐pterygoid resection of a low‐grade cribriform cystadenocarcinoma of the infratemporal fossa
Vikram G. Ramjee, Landon J. Massoth, John P. Richards, Kibwei A. McKinney
World Journal of Otorhinolaryngology - Head and Neck Surgery.2020; 6(2): 115. CrossRef
Psammoma Bodies in a Large Myoepithelioma
Marcela Pessoa de Melo, Diego Filipe Bezerra Silva, Rodrigo Alves Ribeiro, Tony Santos Peixoto, Daliana Queiroga de Castro Gomes, Pollianna Muniz Alves, Cassiano Francisco Weege Nonaka, Bárbara Vanessa de Brito Monteiro
Journal of Craniofacial Surgery.2020; 31(4): e326. CrossRef
Low-grade intraductal carcinoma of salivary glands: A systematic review of this rare entity
Francesco Giovacchini, Caterina Bensi, Stefano Belli, Maria Elena Laurenti, Martina Mandarano, Daniele Paradiso, Michele Giansanti, Antonio Tullio
Journal of Oral Biology and Craniofacial Research.2019; 9(1): 96. CrossRef
What is your diagnosis? Submandibular mass in a dog
Julie Allen, Ashley M. Talley, Carol B. Grindem, Jennifer A. Neel
Veterinary Clinical Pathology.2018; 47(4): 676. CrossRef
Primary acinic cell carcinoma of the lung with psammoma bodies: A case report and review of literature
Xiu-Peng Zhang, Gui-Yang Jiang, Qing-Fu Zhang, Hong-Tao Xu, Qing-Chang Li, En-Hua Wang
Pathology - Research and Practice.2017; 213(4): 405. CrossRef
Cytology of low‐grade cribriform cystadenocarcinoma in salivary glands: Cytological and immunohistochemical distinctions from other salivary gland neoplasms
Yoshiki Ohta, Yuko Hirota, Yohko Kohno, Koji Kishimoto, Tomoko Norose, Nobuyuki Ohike, Masafumi Takimoto, Akira Shiokawa, Hidekazu Ota
Diagnostic Cytopathology.2016; 44(3): 241. CrossRef
Low-grade cribriform cystadenocarcinoma arising from a minor salivary gland: a case report
Masashi Kimura, Shinji Mii, Shinichi Sugimoto, Kosuke Saida, Shojiroh Morinaga, Masahiro Umemura
Journal of Oral Science.2016; 58(1): 145. CrossRef
A Case of Cystadenocarcinoma Arising from Parotid Gland
Jong Chul Hong, Tae Kyoung Koh, Min Gyoung Pak, Heon Soo Park
Korean Journal of Otorhinolaryngology-Head and Neck Surgery.2016; 59(4): 300. CrossRef

Original Articles

MAD2 Expression in Ovarian Carcinoma: Different Expression Patterns and Levels among Various Types of Ovarian Carcinoma and Its Prognostic Significance in High-Grade Serous Carcinoma: Po Eun Park, Ji Yun Jeong, Sun Zoo Kim, Ji Young Park; Korean J Pathol. 2013;47(5):418-425. Published online October 25, 2013; DOI: https://doi.org/10.4132/KoreanJPathol.2013.47.5.418

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Abstract PDF

Background

Mitotic arrest deficiency protein 2 (MAD2) is a key component of spindle assembly checkpoint function, which mediates cell apoptosis through microtubule kinetics. Aberrant expression of MAD2 is believed to be associated with the development of chromosome instability. MAD2 also has a signihicant role in cellular drug resistance to taxane chemotherapeutic agents.

Methods

Expression of MAD2 and p53 was investigated using immunohistochemistry in 85 cases of ovarian carcinomas. Clinicopathological data including progression-free survival were analyzed.

Results

A significant (p=.035) association was observed between the grade of serous carcinoma and the expression level of MAD2. While low-grade serous carcinoma showed a low-level expression of MAD2, high-grade serous carcinoma showed a high-level expression of MAD2. We also determined that low-level expression of MAD2 was associated with reduced progression-free survival (PFS) (p=.016) in high-grade serous carcinoma.

Conclusions

MAD2 expression in ovarian carcinoma is related to the grade of serous carcinoma and PFS of high-grade serous carcinoma. Expression level of MAD2 detected by immunohistochemistry may serve as an indicator in predicting the response of microtubule-interfering chemotherapeutic agents.

Citations

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The role of the MAD2-TLR4-MyD88 axis in paclitaxel resistance in ovarian cancer
Mark Bates, Cathy D. Spillane, Michael F. Gallagher, Amanda McCann, Cara Martin, Gordon Blackshields, Helen Keegan, Luke Gubbins, Robert Brooks, Doug Brooks, Stavros Selemidis, Sharon O’Toole, John J. O’Leary, David Wai Chan
PLOS ONE.2020; 15(12): e0243715. CrossRef
Aneuploidy: Cancer strength or vulnerability?
Giorgia Simonetti, Samantha Bruno, Antonella Padella, Elena Tenti, Giovanni Martinelli
International Journal of Cancer.2019; 144(1): 8. CrossRef
The association between MAD2 and prognosis in cancer: a systematic review and meta-analyses
Tara Byrne, Helen G. Coleman, Janine A. Cooper, W. Glenn McCluggage, Amanda McCann, Fiona Furlong
Oncotarget.2017; 8(60): 102223. CrossRef
Identification of transcription factors (TFs) and targets involved in the cholangiocarcinoma (CCA) by integrated analysis
L Yang, S Feng, Y Yang
Cancer Gene Therapy.2016; 23(12): 439. CrossRef
Proteins of the mitotic checkpoint and spindle are related to chromosomal instability and unfavourable prognosis in patients with myelodysplastic syndrome
Kelly Roveran Genga, Francisco Dário Rocha Filho, Francisco Valdeci de Almeida Ferreira, Juliana Cordeiro de Sousa, Fernando Sergio Studart, Silvia Maria Meira Magalhães, Fabíola Fernandes Heredia, Ronald Feitosa Pinheiro
Journal of Clinical Pathology.2015; 68(5): 381. CrossRef

Comparison of the DNA Preservation in Neutral-Buffered Formalin Fixed Paraffin-Embedded Tissue and in Non-Buffered Formalin Fixed Paraffin-Embedded Tissue.: An Na Seo, Jae Hoon Kim, Dakeun Lee, Ji Yun Jeong, Ji Young Park; Korean J Pathol. 2011;45(6):549-556.; DOI: https://doi.org/10.4132/KoreanJPathol.2011.45.6.549

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Abstract PDF

BACKGROUND
The preservation of optimized DNA and its extraction from formalin-fixed, paraffin-embedded (FFPE) tissues are important issues. There has been some doubt over whether 10% neutral-buffered formalin is an ideal fixation solution for DNA preservation over non-buffered formalin, as conventionally recommended. In this study, the correlation between the efficiency of DNA extraction from FFPE tissues and buffered formalin was evaluated.
METHODS
Several tissues with same conditions except fixatives were fixed in four different formalin solution groups and were routinely processed as paraffin-embedding protocols. DNAs were extracted from four different FFPE tissues that were stored for over 3 months and over 9 months. The quantity and quality of the DNAs were assessed with a NanoDrop ND-1000 spectrophotometer, and the polymerase chain reaction (PCR) amplification and degradation were analyzed via microchip electrophoresis. KRAS mutation analysis and microsatellite instability (BAT25) PCR were performed with each sample.
RESULTS
The results showed no remarkable difference in the four groups.
CONCLUSIONS
The study findings demonstrate that DNA preservation is fairly unaffected by a neutral buffer where there is short formalin manufacture period and an adequate formalin fixation time before embedding in paraffin.

Citations

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Comparison of Direct Sequencing, PNA Clamping-Real Time Polymerase Chain Reaction, and Pyrosequencing Methods for the Detection ofEGFRMutations in Non-small Cell Lung Carcinoma and the Correlation with Clinical Responses to EGFR Tyrosine Kinase Inhibitor
Hyun Ju Lee, Xianhua Xu, Hyojin Kim, Yan Jin, Pingli Sun, Ji Eun Kim, Jin-Haeng Chung
Korean Journal of Pathology.2013; 47(1): 52. CrossRef

Availability of Immunohistochemistry in the Diagnosis of Follicular Variant of Papillary Thyroid Carcinoma.: Ji Yun Jeong, Jung Sik Jang, Yoon Kyung Sohn, Jin Hyang Jung, Yi Kyeong Chun, Ji Young Park; Korean J Pathol. 2010;44(1):48-55.; DOI: https://doi.org/10.4132/KoreanJPathol.2010.44.1.48

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Abstract PDF

BACKGROUND
Making the diagnosis of the follicular variant of papillary thyroid carcinoma (FVPTC) is often difficult, and there are no accurate immunohistochemical or molecular markers. The purpose of this study is to evaluate performing immunohistochemistry to make the diagnosis of FVPTC.
METHODS
A total of 249 thyroid lesions were studied. We made the tissue microarray, and we assessed the expression of HBME-1, galectin-3, CD56, and p63.
RESULTS
Galectin-3, HBME-1, and p63 were positive in 79.7%, 79.7%, and 15.9% of the FVPTC, respectively. These immunohistochemical features of FVPTC were between those of classic papillary thyroid carcinoma (CPTC) and those of non-PTC. The CD56 expression was positive in 75.4% of the FVPTC, which is much higher than that of the CPTC (28.3%), and even higher than that of the non-PTC lesions (60%). Comparing FVPTC with CPTC, the expression of galectin-3 was significantly higher and the expression of CD56 was significantly lower in the CPTCs. Comparing the FVPTC with follicular carcinoma (FC), the expression of all the markers was significantly higher in the FVPTC. Comparing PTC with FC, the expression of CD56 was lower and the expressions of the other markers were higher in the PTCs.
CONCLUSIONS
Galectin-3, HBME-1, and p63 can help make the diagnosis of FVPTC, and a cocktail of these markers can be even more useful. But CD56 is not thought to be useful to make the diagnosis of FVPTC.

Citations

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A Case of Multifocal Papillary Thyroid Carcinoma Consisting of One Encapsulated Follicular Variant withBRAFK601E Mutation and Three Conventional Types withBRAFV600E Mutation
Wook Youn Kim, Young Sin Ko, Tae Sook Hwang, Hye Seung Han, So Dug Lim, Wan Seop Kim, Seo Young Oh
Korean Journal of Pathology.2013; 47(3): 293. CrossRef

Manual Liquid-Based Cytology (Liqui-PREPtrade mark) in Breast Fine Needle Aspiration Cytology: Comparison with the Conventional Smears.: Ji Yun Jeong, Jeong Shik Kim, Young Su Kim, Hye Jung Kim, Ji Young Park; Korean J Cytopathol. 2008;19(1):34-40.; DOI: https://doi.org/10.3338/kjc.2008.19.1.34

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Abstract PDF

Fine needle aspiration (FNA) cytology of the breast is a useful method for diagnosing breast lesions. Yet making the definite diagnosis with performing FNA is limited by some problems, such as the low cellularity, the poor preservation and the obscuring background. Recent studies have found that liquid-based cytology solves such problems, but it is an expensive method and it is limited by the loss of the background information. The purpose of this study is to compare the Liqui-PREP(TM), a new manual liquid-based method of cytology, and the conventional smears for analyzing breast FNA cytology materials. A total of 31 randomized FNA specimens of breast were studied. In each case, both the conventional smears and the Liqui-PREP(TM) method were performed, and the smears were evaluated for cellularity, cellular preservation, the background, the cytologic features and the architectural arrangement. The cellularity and architectural arrangement were equal for both preparations. The Liqui-PREP(TM) specimens showed better cellular preservation, loss of the obscuring background, no overlapping of cells and a smaller area to screen compared with the conventional smears. Moreover, it has the potential advantages of being able to use the remaining specimens for immunohistochemical study and ploidy analysis, and it can reduce the costs for preparation compared with the other liquid-based methods of cytology. But some background information is lost in the Liqui-PREP(TM) specimens, the same as the other liquid-based methods of cytology. In conclusion, the Liqui-PREP(TM) and conventional smears showed good correlation, but they have their respective advantages and disadvantages. These results suggest that Liqui-PREP(TM) can contribute to making the accurate diagnosis with performing breast FNA cytology when it is used along with other methods.

Citations

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Liquid-Based Cytology in Fine-Needle Aspirates of the Thyroid and Breast
Ji-Young Kim
The Korean Journal of Pathology.2009; 43(2): 99. CrossRef
Comparison of Liqui-PREP™ and Conventional Preparations in Thyroid Fine Needle Aspiration
Eun Su Park, Eun Yoon Cho, In Gu Do, Soon Jae Kim, Jung Hee Shin, Boo Kyung Han, Young Lyun Oh
The Korean Journal of Pathology.2009; 43(6): 550. CrossRef

Predictive Factors of Epstein-Barr Virus Association in Gastric Adenocarcinoma.: Young Su Kim, Sang Chul Nam, Man Hoon Han, Ji Yun Jeong, Sun Kyun Park, In Soo Suh, Han Ik Bae; Korean J Pathol. 2008;42(4):193-197.

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Abstract PDF: BACKGROUND
It is important to detect Epstein-Barr virus (EBV) in the setting of gastric cancer so that early viral targeted therapy and prevention can be undertaken. The aim of this study was to investigate the predictive clinicopathologic factors for EBV-related gastric cancer.
METHODS
The archival tumor tissues from 335 patients with gastric cancer were examined using tissue microarray. The detection of EBV was performed using EBV mRNA in situ hybridization (EBV-ISH), and the results were compared against clinicopathologic factors.
RESULTS
EBV-related gastric cancers were identified in 21 of 335 investigated cases (6.27%). The anatomical predisposition of EBV-related cancers to manifest in the upper stomach was statistically significant (p<0.001). EBV-related cancers were almost always (20/21) accompanied by lymphoid stroma. No differences in age, sex, histologic differentiation, or T or N stage were noted between EBV-positive and EBV-negative gastric carcinomas.
CONCLUSIONS
The association of EBV with gastric adenocarcinomas could be predicted when tumors with lymphoid stroma occurred in the upper stomach.

Case Report

Diffuse Ganglioneuromatosis of Appendix associated with Von Recklinghausen;s Disease.: Young Su Kim, Sang Chul Nam, Man Hoon Han, Jeong Shik Kim, Ji Yun Jeong, Han Ik Bae; Korean J Pathol. 2007;41(5):334-336.

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Abstract PDF: Gastrointestinal ganglioneuromatosis is a rare neoplastic condition that can occur in association with von Recklinghausen's disease with multiple endocrine neoplasia type II B. The main locations are the ileum, colon, and appendix. We report a case of diffuse ganglioneuromatosis of the appendix associated with a mesenteric and ileocecal plexiform neurofibroma in von Recklinghausen's disease.

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